For a good separation of a complex mixture of proteins in a wide range of MW, it is usually recommended to use a gel on a gradient of concentration of acrylamide in the length. Offers from Bio-Rad, in the new line of gel Mini GELS PROTEAN ™, a special flavor TGX stretching of the resolution between 10 and 100 kD, where most of the separated proteins present in electrophoresis.Even with a special uniform, chemical acrylamide% generates this model.This represents a kD gel is a good option for optimal separation in that range.See in Figure 1 comparison resolution of a homogenate of e. Coli in a kD and a gel of functioning of TGX each individual 4-20%. Please note that this special gel, if all TGX gels, a useful life of 12 months, compatible with the standard SDS running buffer Tris-glicina-Laemmli has faster, to 10 minutes for the mini size you can make.
